Method for purifying plasmid dna and plasmid dna...

C - Chemistry – Metallurgy – 12 – N

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C12N 1/06 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2311600

A method is described for purifying plasmid DNA from a mixture containing plasmid DNA and genomic DNA. A solution containing both plasmid DNA and genomic DNA is treated with at least 80 % by weight saturation ammonium sulfate, thereby precipitating the genomic DNA and providing purified plasmid DNA in solution. Genomic DNA levels in the purified plasmid DNA product are less than 1 % by weight based on the plasmid DNA. The purified plasmid DNA is suitable for use in humans.

La présente invention concerne un procédé de purification d'ADN plasmidique contenu dans un mélange renfermant de l'ADN plasmidique et de l'ADN génomique. Une solution contenant à la fois de l'ADN plasmidique et de l'ADN génomique est traitée avec au moins 80 % en poids de sulfate d'ammonium de saturation, induisant ainsi la précipitation de l'ADN génomique et maintenant l'ADN plasmidique purifié dans la solution. Les taux d'ADN génomique dans le produit d'ADN plasmidique purifié sont inférieurs à 1 % en poids, sur la base de l'ADN plasmidique. L'ADN plasmidique purifié peut être utilisé chez les humains.

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