Method for generation of sequence sampled maps of complex...

Details Method for generation of sequence sampled maps of complex... Method for generation of sequence sampled maps of complex...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07K 14/575 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2164004

The present invention relates to a rapid and powerful sequence "sequence sampled mapping" method for sequencing complex genomes. The invention method is applicable to genomic DNA, preferably mammalian chromosomes, and in a preferred embodiment, employs a "bottom-up" mapping strategy, which allows for the simultaneous analysis of multiple cosmid clones for the detection of overlaps. The sequence sample mapping method is useful first, for the completion of high density , based maps, and ultimately, for the complete sequencing of genomic DNA directly from cosmid clones.

Procédé rapide et performant permettant d'établir des cartes par échantillonnage de séquences pour le séquençage de génomes complexes. Le procédé s'applique à l'ADN génomique, de préférence aux chromosomes mammifères, et dans un mode préféré de réalisation, il met en oeuvre une stratégie "ascendante" d'établissement de cartes, ce qui permet l'analyse simultanée de plusieurs clones de cosmide et la détection des recouvrements. Ce procédé d'établissement de cartes par échantillonnage de séquences est utilisable dans un premier temps dans la préparation de cartes à base de séquences à densité élevée, et plus tard dans le séquençage total de l'ADN génomique directement à partir des clones de cosmide.

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