Pluripotent rabbit embryonic stem (es) cell lines and their...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/06 (2006.01) A01K 67/027 (2006.01) C12N 15/85 (2006.01) C12N 15/87 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2252524

The present invention relates to a rabbit embryonic stem (ES) cell line, comprising at least 70 %, preferably 80 to 90 % undifferentiated cells and obtainable by isolating the inner cell mass of 5.5 days postcoitus blastocysts and culturing them on feeder cells in rabbit ES medium. The invention further relates to further optimization of derivation and maintenance of the cell line and to the use thereof in inter alia generation of chimeric rabbits.

Lignée de cellules souches embryonnaires de lapin, qui comprend au moins 70 %, de préférence 80 à 90 %, de cellules indifférenciées et obtenues par isolement de l'amas cellulaire interne de blastocystes 5,5 jours postcoïtus et par culture desdites lignées sur des cellules nourricières dans un milieu pour cellules souches embryonnaires de lapin. La présente invention concerne en outre l'optimisation de la dérivation et de la conservation de ladite lignée cellulaire et l'utilisation de cette dernière entre autres pour produire des lapins chimériques.

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