Use of arabinogalactan in cell cryopreservation media

A - Human Necessities – 01 – N

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A01N 1/02 (2006.01)

Patent

CA 2250084

Methods and compositions for cryopreserving somatic cells are provided, In one embodiment, a cell cryopreservation medium is provided which includes an effective amount of arabinogalactan to maintain the viability of cells upon freezing, storage and thawing. The cells may be cooled or frozen during storage to a temperature about or below 4°C, for example, to about - 196°C. In one preferred embodiment, ultrarefined arabinogalactan is provided in the cryopreservation medium, optionally in combination with a second cryopreservation agent, such as dimethyl sulfoxide. The medium can be used for the cryopreservation of a wide variety of different cell types from different sources. For example, mammalian cells, including porcine, canine, human, equine, rodent and bovine cells can be cryopreserved in the medium. The presence of arabinogalactan in the medium protects the viability of cells in the medium during the process of freezing, storage and thawing.

Méthodes et compositions pour la cryoconservation des cellules somatiques. Dans un modèle, un milieu de cryoconservation des cellules inclut une quantité efficace d'arabinogalactane pour maintenir la viabilité des cellules lors de la congélation, de l'entreposage et de la décongélation. Les cellules peuvent être refroidies ou congelées durant l'entreposage à une température d'environ 4 degrés C ou moins, par exemple, jusqu'à environ 196 degrés C. Dans le modèle privilégié, de l'arabinogalactane ultra-raffiné est présent dans le milieu de cryoconservation, associé parfois à un second agent de cryoconservation, comme le sulfoxyde de diméthyle. Le milieu peut être utilisé pour la cryoconservation d'une grande variété de cellules de types différents provenant de différentes sources. Par exemple, des cellules de mammifères, notamment de porcins, de chiens, d'humains, de chevaux, de rongeurs et de bovins, peuvent être cryoconservées dans le milieu. La présence d'arabinogalactane préserve la viabilité des cellules dans le milieu durant la congélation, l'entreposage et la décongélation.

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