Recombinant baculovirus and use thereof in the production of...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/866 (2006.01) C07K 16/00 (2006.01) C12N 5/10 (2006.01) C12N 7/01 (2006.01) C12N 15/13 (2006.01) C12N 15/86 (2006.01) C12P 21/08 (2006.01)

Patent

CA 2182380

Recombinant baculovirus used as an expression vector in the production of immunoglobulins within an insect cell. The invention is characterized by an expression cassette comprising a sequence coding for at least one portion of an immunoglobin H chain, said sequence being transcriptionally controlled by a first baculovirus promotor, and an expression cassette comprising a sequence coding for at least one portion of an immunoglobin L chain, said sequence being transcriptionally controlled by a second baculovirus promotor. The first and second promotors are two different promotors or derivatives of different promotors, the first and second promotors residing in different loci.

L'invention est relative à un baculovirus recombinant, constituant un vecteur d'expression utilisable pour la production d'immunoglobulines dans une cellule d'insecte, et caractérisé en ce qu'il comprend: une cassette d'expression comprenant une séquence codant pour au moins une partie d'une chaîne H d'une immunoglobine, laquelle séquence est placée sous contrôle transcriptionnel d'un premier promoteur de baculovirus; et une cassette d'expression comprenant une séquence codant pour au moins une partie d'une chaîne L d'une immunoglobuline, laquelle séquence est placée sous contrôle transcriptionnel d'un second promoteur de baculovirus; le premier et le second promoteurs étant deux promoteurs différent ou des dérives de promoteurs différent et le premier et le second promoteurs étant situés dans des locus différents.

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