Methods for identifying compounds that modulate untranslated...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) A61K 45/00 (2006.01) A61P 9/10 (2006.01) A61P 35/00 (2006.01) C12Q 1/00 (2006.01) C12Q 1/02 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2514184

The present invention relates to methods for identifying compounds that modulate untranslated region-dependent expression of a target gene. The invention particularly relates to using untranslated regions of a target gene or fragments thereof linked to a reporter gene to identify compounds that modulate untranslated region-dependent expression of a target gene. The methods of the present invention provide a simple, sensitive assay for high- throughput screening of libraries of compounds to identify pharmaceutical leads.

La présente invention concerne des procédés permettant d'identifier des composés qui modulent l'expression liée à une région non traduite d'un gène cible. L'invention concerne plus particulièrement l'utilisation de régions non traduite d'un gène cible ou de certains de ses fragments liés à un gène rapporteur pour identifier des composés qui modulent l'expression dépendant d'une région non traduite d'un gène cible. Les procédés de la présente invention constituent un essai simple et sensible pour la recherche systématique à débit élevé d'échantillothèques de composés pour identifier des filières pharmaceutiques.

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