Process for modifying the stability of antibodies

Details Process for modifying the stability of antibodies Process for modifying the stability of antibodies

C - Chemistry, Metallurgy

12

N

C12N 15/13 (2006.01) C07K 16/00 (2006.01) C07K 19/00 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2195076

Process for the production of a functional immunoglobulin or functional derivative or fragment thereof with an improved stability in a eukaryotic or prokaryotic organism by transformation with an expression vector which contains a recombinant gene. which codes for the said immunoglobulin derivative or fragment which is characterized in that a modified gene is used which contains at least one codon which is substituted compared to the unmodified gene and codes for a more frequent amino acid. In this way it is also possible to produce active antibodies free of disulfide bridges. In a modification of the process it is possible to selectively destabilize antibodies.

L'invention concerne un procédé de préparation d'une immunoglobuline fonctionnelle ou d'un dérivé fonctionnel ou d'un fragment d'immunoglobiline, à stabilité améliorée dans un organisme eucaryote ou procaryote, après transformation avec un vecteur d'expression renfermant un gène de recombinaison codant pour le dérivé ou fragment d'immunoglobuline précité, caractérisé en ce qu'on utilise un gène modifié, dans lequel, par rapport au gène non modifié, au moins un codon codant pour un aminoacide plus fréquent est remplacé. De cette façon, des anticorps actifs, exempts de ponts disulfure peuvent également être préparés. Selon une variante du procédé, des anticorps peuvent être déstabilisés de façon adéquate.

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