Fluorescent proteins

Details Fluorescent proteins Fluorescent proteins

C - Chemistry, Metallurgy

12

N

C12N 15/12 (2006.01) C07K 14/435 (2006.01) C07K 19/00 (2006.01) C12N 15/09 (2006.01) C12N 15/65 (2006.01) C12P 21/00 (2006.01) G01N 33/533 (2006.01)

Patent

CA 2410413

A GFP with an F64L mutation and an E222G mutation is provided. This GFP has a bigger Stokes shift compared to other GFPs making it very suitable for high throughput screening due to a better resolution. This GFP also has an excitation maximum between the yellow GFP and the cyan GFP allowing for cleaner band separation when used together with those GFPs.

L'invention concerne une GFP (protéine fluorescente verte) comprenant une mutation F64L et une mutation E222G. Cette GFP possède un décalage Stokes plus important que celui d'autres GFP, rendant cette protéine très appropriée à un criblage à rendement élevé, par suite d'une meilleure résolution. De même, cette GFP comprend un maximum d'excitation se situant entre la GFP jaune et la GFP cyan, qui permet une séparation de bande plus nette lorsqu'elle est utilisée ensemble avec ces GFP.

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