Cell culture method and utilization of the same

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 5/00 (2006.01) C12N 5/10 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2593521

It is intended to make cells to produce a protein at a high level by using a medium containing an enzymatically digested product of fish meant or a fish meat extract. Namely, a method of culturing cells characterized by comprising initiating the culture of the cells in a medium containing an enzymatically digested product of fish meant or a fish meat extract, and then adding the enzymatically digested product of fish meant or the fish meat extract to the medium at least once during the cell culture; and a method of producing a desired protein by using this culture method.

La présente invention décrit l'élaboration de cellules produisant une protéine à des taux élevés, en employant un milieu de culture contenant un produit de digestion enzymatique issu de chair de poisson, ou un extrait de chair de poisson. La présente invention décrit notamment une méthode de culture cellulaire caractérisée en ce qu'elle comprend les étapes suivantes : mise en AEuvre de la culture des cellules dans un milieu de culture contenant un produit de digestion enzymatique issu de chair de poisson ou un extrait de chair de poisson, puis ajout dudit produit ou dudit extrait au milieu au moins une fois au cours de la culture. La présente invention décrit en outre une méthode de production d'une protéine recherchée par emploi de ladite méthode de culture.

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