Process for protein extraction

Details Process for protein extraction Process for protein extraction

C - Chemistry, Metallurgy

07

K

C07K 1/36 (2006.01) C07K 14/245 (2006.01)

Patent

CA 2497209

The invention includes a process for extracting a target protein from E.coli cells that includes lowering the pH of a whole E. coli cell solution to form an acidic solution, disrupting the cells to release the protein into the acidic solution, and separating the cellular debris from the released protein to obtain a protein product enriched in the heterologous target protein. The invention also includes addition of a solubility enhancer.

La présente invention a trait à un procédé permettant l'extraction d'une protéine cible à partir de cellules d'E.coli comprenant la réduction du pH d'une solution cellulaire d'E.colientière pour former une solution acide, la dissociation des cellules pour libérer la protéine dans la solution acide, et la séparation des débris cellulaires de la protéine libérée pour obtenir un produit protéique enrichi par la protéine cible hétérologue. L'invention comporte également l'addition d'un activateur de solubilité.

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