Multiplex cytokine analysis

G - Physics – 01 – N

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G01N 33/68 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2385614

The invention provides methods and compositions for the simultaneous, quantitative detection of cytokines in a sample. The methods use a solid phase array comprising a plurality of different antibodies arrayed in corresponding discrete array elements and specific for a corresponding plurality of different cytokines. The invention also provides methods of manufacturing and using the arrays for the simultaneous, quantitative detection of cytokines in samples.

La présente invention concerne des procédés et des compositions destinées à la détection quantitative, simultanée, de cytokines dans un échantillon. Les procédés mettent en oeuvre une matrice à phase solide comprenant plusieurs anticorps, placés dans des éléments discrets de la matrice, et qui sont spécifiques vis à vis de plusieurs cytokines différentes. L'invention concerne aussi des procédés de fabrication et d'utilisation des matrices destinées à la détection quantitative et simultanée de cytokines dans des échantillons.

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