Dna polymerase having ability to reduce innate selective...

Details Dna polymerase having ability to reduce innate selective... Dna polymerase having ability to reduce innate selective...

C - Chemistry, Metallurgy

12

N

C12N 9/22 (2006.01) C07K 14/195 (2006.01) C12N 9/12 (2006.01) C12N 15/55 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2344105

The invention relates to genetical modification of DNA polymerase to reduce its innate selective sequence-related discrimination against incorporation of fluorescent dye-labeled ddCTP and ddATP in the enzymatic reaction for preparation of samples for automated fluorescent dye-labeled terminator DNA sequencing. The modified DNA polymerases are more resistant to heat inactivation and are more effective in dideoxynucleotide incorporation than current DNA polymerases.

L'invention concerne une modification génétique d'ADN polymérase de manière que soit supprimée la discrimination relative à la séquence sélective de l'innate, contre l'incorporation de ddCTP et ddATP marquées au colorant fluorescent, dans la réaction enzymatique pour la préparation d'échantillons de séquençage automatisé d'ADN de terminaison marquée par un colorant fluorescent. Lesdites ADN polymérases modifiées sont plus résistantes à l'inactivation thermique et sont plus efficaces dans l'incorporation de didésoxynucléotides que les ADN polymérases courantes.

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