Fluorescence-based electrophoresis system for polynucleotide...

G - Physics – 01 – N

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G01N 27/447 (2006.01) G01N 21/64 (2006.01)

Patent

CA 2179714

The present invention relates to a fluorescent polynucleotide analyzer system which utilizes electrophoresis and in particular, capillary- based electrophoresis. The analyzer system of the present invention is comprised of, in combination, a capillary containing an electrophoretic separation medium, a means for introducing a mixture of fluorescently labeled polynucleotide fragments into the capillary, detection means for detecting tile fluorescently labeled polynucleotide fragments, including the simultaneous detection of multiple fluorescent labels, and, means for introducing and removing electrophoretic separation medium from the capillary.

La présente invention concerne un système d'analyse de polynucléotides fluorescents qui fait appel à l'électrophorèse, et en particulier l'électrophorèse capillaire. Ce système d'analyse comporte, en association, un capillaire contenant un milieu de séparation électrophorétique, un organe pour introduire dans le capillaire un mélange de fragments de polynucléotides à marqueurs fluorescents, un organe permettant de détecter lesdits fragments de polynucléotides, notamment la détection simultanée de marqueurs fluorescents multiples, et des organes permettant d'introduire dans le capillaire un milieu de séparation électrophorétique et de l'en retirer.

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