Method for immobilizing substances

G - Physics – 01 – N

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G01N 33/543 (2006.01) C12Q 1/68 (2006.01) G01N 33/544 (2006.01) G01N 33/547 (2006.01)

Patent

CA 2390158

A method for immobilizing a material comprising placing a material to be immobilized on the surface of a support and then forming a plasma-polymerized film; and an immobilized material which has been immobilized by using the method. Holding a material to be immobilized utilizing a plasma-polymerized film leads to a significant reduction in the damage to the material and also the liberation of the material from the support. Further, the immobilized material is present on the resultant surface of the support and maintains its bonding activity, which results in the application of the method, for example, to the indirect immobilization of biotinilated polynucleotide and to the detection of a ligand through immobilization of a protein capable of bonding such as an antibody.

L'invention concerne une méthode d'immobilisation d'une matière consistant à placer une matière à immobiliser sur la surface d'un support et ensuite à former une couche mince polymérisée au plasma, ainsi qu'une matière immobilisée ayant été immobilisé à l'aide de la méthode. Le maintien d'une matière à immobiliser à l'aide d'une couche mince polymérisée au plasma conduit à une réduction significative de la détérioration de la matière et également à la libération de la matière du support. De plus, la matière immobilisée est présente sur la surface résultante du support et maintient son activité de liaison, ce qui se traduit par l'application de la méthode, par exemple, à l'immobilisation indirecte d'un polynucléotide biotinilé ainsi qu'à la détection d'un ligand par immobilisation d'une protéine capable de fixer un tel anticorps.

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