Recombinant cellulases

Details Recombinant cellulases Recombinant cellulases

C - Chemistry, Metallurgy

12

N

C12N 15/56 (2006.01) C12N 1/15 (2006.01) C12N 1/21 (2006.01) C12N 9/24 (2006.01) C12N 9/42 (2006.01)

Patent

CA 2139099

A method of cloning of cellulase cDNA clones with enzymatical activity from an anaerobic rumen fungus including the steps of: (i) cultivation of an anaerobic rumen fungus; (ii) isolating total RNA from the culture in step (i); (iii) isolating poly A+ mRNA from the total RNA referred to in step (ii); (iv) constructing a cDNA expression library; (v) ligating cDNA to a bac- teriophage expression vector selected from .lambda.ZAP, .lambda.ZAPII or vectors of similar properties; (vi) screening of cellulase positive recombinant clones in a culture medium incorporating cellulose by detection of cellulose hydrolysis; and (vii) purifying cellulase positive recombinant clones. There is also provided recombinant cellulase fungal cDNA clones produced by the abovementioned method as well as the recombinant cellulase cDNA clones, derived from N. patriciarum, having the property of production of bi- ologically functional cellulases in E. coli cells. There is also provided various cDNA molecules which may be utilised in the abovementioned method.

Affiliated with

Also associated with

Rating

Recombinant cellulases does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Recombinant cellulases, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Recombinant cellulases will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1544634