C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01) B01D 15/00 (2006.01) C07B 61/00 (2006.01) C07H 1/08 (2006.01) C07H 21/00 (2006.01)
Patent
CA 2428532
A method is provided for using a silanized silica matrix to isolate a target nucleic acids, such as plasmid DNA, fragments of DNA, chrmosomal DNA, or RNA from contaminants, including proteins, lipids, cellular debris, or non-target nucleic acids. The silanized silica matrix comprises a silica based solid phase and a plurality of silane ligands covalently attached to the surface of the solid phase. Non-target material absorbs to the silanized silica matrix in the presence of a sufficient concentration of chaotropic salt, while target nucleic acids adsorb to the matrix under other solution conditions. The method of using the sinalized silica matrix of the present invention can be used to clear solutions of disrupted biological material, and to isolate nucleic acids therefrom or from other solutions containing nucleic acids and at least one contaminant.
L'invention concerne un procédé d'utilisation de matrice de silice silanisée en vue d'isoler un acide nucléique recherché, par exemple ADN plasmidique, fragments d'ADN, ADN chromosomique ou ARN de contaminants, incluant protéines, lipides, débris cellulaires ou acides nucléiques non recherchés. La matrice de silice silanisée comporte une phase solide à base de silice et une pluralité de ligands de silane fixés de manière covalente à la surface de la phase solide. La matière non recherchée absorbe la matrice de silice silanisée en présence d'une concentration suffisante de sel chaotropique, et les acides nucléiques recherchés sont adsorbés sur la matrice dans d'autres conditions de solution. Ce procédé d'utilisation de la matrice de silice silanisée peut servir à purifier des solutions de matières biologiques dissociées, et à isoler des acides nucléiques de ces solutions ou d'autres solutions contenant des acides nucléiques et au moins un contaminant.
Bitner Rex M.
Flemming Roderick G.
Koller Susan C.
Simpson Daniel J.
Promega Corporation
Torys Llp
LandOfFree
Lysate clearance and nucleic acid isolation using silanized... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Lysate clearance and nucleic acid isolation using silanized..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Lysate clearance and nucleic acid isolation using silanized... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1547475