An inducible method for production of recombinant...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/86 (2006.01) C12N 7/01 (2006.01) C12N 15/864 (2006.01)

Patent

CA 2264482

Methods for efficient production of recombinant AAV are described. In one aspect, three vectors are introduced into a host cell. A first vector directs expression of T7 polymerase. A second vector carries rep and cap under the control of the T7 promoter. A third vector contains a rAAV cassette which contains a minigene flanked by AAV ITRs. In a second aspect, the host cell is stably transfected to contain a plasmid bearing one of the required vector components and the host cell is double transfected/infected.

L'invention concerne des procédés de production efficace de Vaa recombinés. Dans un premier aspect de l'invention, trois vecteurs sont introduits dans une cellule hôte. Un premier vecteur dirige l'expression de la polymérase T7. Un deuxième vecteur porte des gènes rep et cap sous le contrôle du promoteur T7. Un troisième vecteur contient une cassette de Vaa recombinés qui comporte un minigène flanqué de RTi de Vaa adjacents. Dans un second aspect de l'invention, la cellule hôte est transfectée de façon stable de sorte qu'elle contient un plasmide renfermant un des constituants vectoriels appropriés et la cellule hôte est doublement transfectée/infectée.

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