Compositions, methods, and devices for isolating biological...

Details Compositions, methods, and devices for isolating biological... Compositions, methods, and devices for isolating biological...

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C12Q 1/24 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2685209

Compositions, methods, devices, and kits, which include an immobilized-metal support material comprising a substrate having a plurality of -C(O)O- or -P(O)(-OH)2-x(-O-)x groups bound to the substrate and a plurality of metal ions, My+, bound to the -C(O)O- or -P(O)(-OH)2-x(-O-)x groups; wherein M is selected from the group consisting of zirconium, gallium, iron, aluminum, scandium, titanium, vanadium, yttrium, and a lanthanide; y is an integer from 3 to 6; and x is 1 or 2, and to which microorganisms and polynucleotides bind, and which can be used for separating and optionally assaying microorganisms and/or a polynucleotide from a sample material are disclosed.

La présente invention concerne des compositions, des méthodes, des dispositifs et des trousses qui comprennent un matériau support à métal immobilisé comprenant un substrat auquel une pluralité de groupes -C(O)O- ou -P(O)(-OH)2-x(-O-)x est liée, une pluralité d'ions métalliques My+ étant liée auxdits groupes -C(O)O- ou -P(O)(-OH)2-x(-O-)x; M étant sélectionné dans le groupe composé de zirconium, gallium, fer, aluminium, scandium, titane, vanadium, yttrium et d'un lanthanide; y représentant un nombre entier compris entre 3 et 6; et x désignant 1 ou 2. Des microorganismes et des polynucléotides se lient au matériau support à métal immobilisé, lequel peut être utilisé pour séparer et éventuellement analyser des microorganismes et/ou un polynucléotide à partir d'un matériel échantillon.

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