Methods for increasing starch content in plants

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/82 (2006.01) C12N 9/00 (2006.01)

Patent

CA 2704016

Methods and compositions for increasing the starch content in green tissues of a plant are provided. The method comprises down-regulating the activity of starch degradation enzymes in a plant. The resulting transgenic plants of the invention have increased starch content in green tissues and exhibit a starch excess phenotype. In one embodiment the method involves manipulating a monocot plant to down-regulate the activity of a starch degradation enzyme. The plants are useful for improving the yield of free sugars from plant biomass and increase dried green tissue storage stability.

L'invention concerne des méthodes et des compositions destinées à augmenter la teneur en amidon des tissus verts d'un végétal. La méthode consiste à réguler à la baisse l'activité d'enzymes de dégradation d'amidon dans une plante. Les plantes transgéniques résultantes selon l'invention présentent une teneur accrue en amidon dans les tissus verts, ainsi qu'un phénotype d'excédent d'amidon. Dans un mode de réalisation, la méthode consiste à manipuler une plante monocotylédone pour réguler à la baisse l'activité d'une enzyme de dégradation d'amidon. Les plantes selon l'invention permettent d'augmenter le rendement de sucres libres provenant de la biomasse végétale et d'améliorer la stabilité au stockage de tissus verts séchés.

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