Corrective method for processing the results of...

G - Physics – 06 – F

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G06F 19/00 (2006.01)

Patent

CA 2672943

The invention relates to a corrective method for processing the results of transcriptomic experiments obtained by differential analysis, wherein said method comprises the following steps: obtaining results of the gene expression level in reference conditions and calculating the average expression level of each of said genes; obtaining results of the gene expression level in processing conditions and calculating the average expression level of each of said genes; calculating the modulation coefficient of the expression level for each of said genes; calculating a p-value associated with each modulation coefficient; and calculating p-value isobar curves according to the average expression level of each of said genes in the reference condition; calculating and associating a medium modulation coefficient on the isobar curve of each p-value observed. The invention can particularly be used for processing the results of experiments on DNA chips.

L'invention concerne une méthode correctrice de traitement de résultats d'expériences transcriptomiques obtenus par analyse différentielle. L'invention qui comporte les étapes suivantes: on obtient des résultats du niveau d'expression de gènes dans une condition de référence, et on calcule le niveau moyen d'expression de chacun desdits gènes; on obtient des résultats du niveau d'expression desdits gènes dans une condition de traitement, et on calcule le niveau moyen d'expression pour chacun desdits gènes; on calcule le coefficient de modulation du niveau d'expression pour chacun desdits gènes; on calcule une p-value associée à chaque coefficient de modulation; et on calcule des courbes isobares de p-value en fonction du niveau moyen d'expression de chacun desdits gènes dans la condition de référence; on calcule et on associe un coefficient de modulation médian sur la courbe isobare de chaque p- value observée. L'invention s'applique en particulier au traitement de résultats d'expériences pratiquées sur des puces à ADN.

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