Improved nucleic acid amplification procedure

Details Improved nucleic acid amplification procedure Improved nucleic acid amplification procedure

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2621267

The invention provides methods for amplification of polynucleotide sequences using primers containing single-stranded RNA. The methods employ use of an enzyme capable of cleaving single-stranded RNA, such as RNase I, to degrade a first RNA- containing primer prior to addition of a second RNA-containing primer. The invention also provides compositions and kits for practicing the amplification methods, as well as methods which use the amplification products.

L'invention concerne des méthodes d'amplification de séquences polynucléotidiques contenant un ARN simple brin. Lesdites méthodes font appel à l'utilisation d'une enzyme pouvant cliver un ARN simple brin, tel que la RNase I, afin de dégrader une première amorce contenant un ARN avant l'addition d'une seconde amorce contenant un ARN. L'invention concerne également des compositions et des trousses pour la mise en pratique des méthodes d'amplification, ainsi que des méthodes qui font appel aux produits d'amplification.

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