Mammalian expression systems

Details Mammalian expression systems Mammalian expression systems

C - Chemistry, Metallurgy

12

P

C12P 21/02 (2006.01) A61K 38/00 (2006.01) C12N 5/10 (2006.01) C12N 15/00 (2006.01) C12N 15/67 (2006.01) C12N 15/85 (2006.01) C07K 14/71 (2006.01)

Patent

CA 2605038

The present invention features mammalian expression systems with improved production yields, and method of using these systems to produce desired proteins. In one embodiment, the expression systems of the present invention comprise genetically-engineered mammalian host cells cultured in a medium that contains an effective amount of heparin or heparin-like molecules. The presence of heparin or heparin-like molecules significantly increases protein production by the cultured cells. The present invention also features the use of constitutively-active components of FGFR-I -mediated signal transduction pathways to improve protein production by cultured mammalian cells. Co- expression of such a component with a protein of interest markedly increases the production yield of the protein of interest.

L'invention concerne des systèmes d'expression chez les mammifères à rendements améliorés et un procédé d'utilisation de ces systèmes afin d'obtenir des protéines souhaitées. Dans un mode de réalisation, les systèmes d'expression de cette invention comprennent des cellules hôtes de mammifère génétiquement modifiées cultivées dans un milieu contenant une quantité efficace de molécules d'héparine ou de type héparine. La présence de ces molécules augmente considérablement la production de protéines au moyen de la culture de cellule. L'invention concerne également l'utilisation de composants à constitution active de voies de transduction de signal induite par FGFR-I afin d'améliorer la production de protéines par la culture de cellules de mammifère. La co-expression d'un tel composant avec la protéine d'intérêt augmente énormément le rendement de la protéine d'intérêt.

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