C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2294962
The present invention relates to a method for determining the sequence of a polynucleotide, the method comprising the steps of: (i) reacting a target polynucleotide with a polymerase enzyme immobilised on a solid support, and the different nucleotides, under conditions sufficient for the polymerase reaction; and (ii) detecting the incorporation of a specific nucleotide complementary to the target polynucleotide, by measuring radiation.
L'invention concerne un procédé pour déterminer la séquence d'un polynucléotide, qui consiste : (1) à faire réagir un polynucléotide cible avec une enzyme polymérase immobilisée sur un support solide, et à introduire les différents nucléotides, dans des conditions suffisantes pour assurer la réaction avec la polymérase; et (2) à déceler l'incorporation d'un nucléotide spécifique complémentaire dans le polynucléotide cible, en effectuant des mesures de rayonnement.
Cassan Maclean
Gen-Probe Incorporated
Medical Biosystems Ltd.
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