Method of analyzing protein using laser ablation

G - Physics – 01 – N

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G01N 27/64 (2006.01) G01N 21/55 (2006.01) G01N 21/71 (2006.01) G01N 33/68 (2006.01) H01J 49/04 (2006.01) H01J 49/10 (2006.01)

Patent

CA 2495798

A method of analyzing a protein using laser ablation which comprises, in order to simultaneously atomize the atoms constituting the protein to be analyzed and ionize the same using a single laser source, irradiating the protein with laser beams and thus ablating the protein to thereby atomize the protein into the constituting elements and ionize the thus atomized constituting elements, and then analyzing the constituting elements thus ionized. The laser beams for ablating the subject protein are ultra-short pulse laser beams with which a chip having the protein fixed thereon is irradiated. Thus, the protein fixed on the chip is ablated with the ultra-short pulse laser beams so that the protein is atomized into the constituting elements and, at the same time, ionized followed by the analysis of the constituting elements thus ionized.

Procédé d'analyse d'une protéine à l'aide de l'ablation au laser, qui consiste, afin d'atomiser simultanément les atomes constituant la protéine à analyser et d'ioniser lesdits atomes à l'aide d'une seule source laser, à exposer la protéine à des faisceaux laser et donc à procéder à une ablation de ladite protéine pour atomiser la protéine en ses éléments constitutifs et ioniser les éléments constitutifs ainsi atomisés, puis à analyser les éléments constitutifs ainsi ionisés. Les faisceaux laser servant à l'ablation de la protéine analysée sont des faisceaux laser à impulsions ultracourtes auxquels est exposée une puce sur laquelle est fixée la protéine. Par conséquent, la protéine fixée sur la puce subit une ablation à l'aide de faisceaux laser à impulsions ultracourtes si bien que ladite protéine est atomisée en ses éléments constitutifs et en même temps ionisée, les éléments constitutifs ainsi ionisés étant alors analysés.

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