Electrophoresis separation and treatment of samples

B - Operations – Transporting – 01 – D

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B01D 57/02 (2006.01) C25B 7/00 (2006.01) G01N 27/447 (2006.01)

Patent

CA 2405033

An electrophoresis system for separating small macromolecules comprising two electrophoretic systems, wherein each system comprises an anode buffer and cathode buffer chamber containing an anode and cathode therein; an ion- permeable separation membrane positioned between the anode and cathode buffer chambers; an ion-permeable restriction membrane positioned either side of the ion-permeable separation membrane to define first and second interstitial volumes; an electric field applied between the buffer chambers, and wherein the two electrophoretic systems are in fluid communication with each other.

L'invention se rapporte à un système d'électrophorèse conçu pour séparer de petites macromolécules et comportant deux systèmes électrophorétiques comprenant chacun une chambre à tampon anodique et une chambre à tampon cathodique contenant une anode et une cathode; une membrane de séparation perméable aux ions et positionnée entre les chambres tampons anodique et cathodique; et une membrane de restriction perméable aux ions positionnée de part et d'autre de la membrane de séparation perméable aux ions de manière à définir un premier et un second volume interstitiel. Un champ électrique est appliqué entre les chambres tampons et les deux systèmes électrophorétiques sont en communication fluidique l'un avec l'autre.

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