Method for improved single cell cloning

Details Method for improved single cell cloning Method for improved single cell cloning

C - Chemistry, Metallurgy

12

N

C12N 5/02 (2006.01) C12N 5/10 (2006.01)

Patent

CA 2756247

The present invention relates to methods for the cultivation of a population of cells in a serum free cell culture medium, wherein the population of cells has a cell concentration of less than 100 cells/ml, wherein a serum free cell culture medi-um containing recombinant albumin and recombinant transferrin is used.

La présente invention concerne des procédés pour la culture d'une population de cellules dans un milieu de culture de cellules sans sérum, la population de cellules ayant une concentration de cellules de moins de 100 cellules/ml, un milieu de culture de cellules sans sérum contenant de l'albumine recombinante et de la transferrine recombinante étant utilisé.

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