System and method for increasing the contrast of an image...

G - Physics – 02 – B

Patent

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G02B 21/00 (2006.01) G02B 21/04 (2006.01) G02B 21/06 (2006.01) G02B 21/16 (2006.01) G02B 21/34 (2006.01)

Patent

CA 2441878

The contrast of an image produced by epifluorescence microscopy may be increased by placing a high-pass dichroic reflecting film behind the sample. The reflecting film reflects the emission light emitted by the fluorescent tags in the sample back through the objective lens while allowing the shorter wavelength excitation light to pass through the sample holder.

On peut accentuer le contraste d'une image produite par microscopie à épifluorescence en plaçant un film réfléchissant dichroïque passe-haut derrière l'échantillon. Ce film réfléchissant reflète en retour la lumière émise par les marqueurs fluorescents de l'échantillon à travers l'objectif tout en laissant la lumière d'excitation, de longueur d'onde plus courte, traverser le porte-échantillon.

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