Highly simplified lateral flow-based nucleic acid sample...

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G01N 1/00 (2006.01) C12Q 1/68 (2006.01) G01N 1/40 (2006.01) G01N 33/543 (2006.01) G01N 33/567 (2006.01) C12M 1/40 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2723536

Highly simplified lateral flow chromatographic nucleic acid sample preparation methods, devices, and integrated systems are provided for the efficient concentration of trace samples and the removal of nucleic acid amplification inhibitors. Methods for capturing and reducing inhibitors of nucleic acid amplification reactions, such as humic acid, using polyvinylpyrrolidone treated elements of the lateral flow device are also provided. Further provided are passive fluid control methods and systems for use in lateral flow assays.

Linvention propose des procédés de préparation déchantillon dacide nucléique chromatographique à écoulement latéral hautement simplifiés, des dispositifs et systèmes intégrés pour la concentration efficace déchantillons à létat de traces et lélimination dinhibiteurs damplification dacide nucléique. Linvention propose également des procédés pour capturer et réduire des inhibiteurs de réactions damplification dacide nucléique, tels que lacide humique, au moyen déléments traités par polyvinylpyrrolidone du dispositif découlement latéral, ainsi que des procédés de régulation de fluide passive et des systèmes à utiliser dans des essais à écoulement latéral.

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