Device and process for assays using binding members

Details Device and process for assays using binding members Device and process for assays using binding members

B - Operations, Transporting

01

J

B01J 19/00 (2006.01) B01L 3/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2668639

A device comprising a rigid substrate, a flexible cover element at least partially covering the substrate, a first structure formed in the substrate, adapted for accommodating liquids and adapted for releasing contents of one or more cells, spores, or viruses, the contents including the target molecules, a second structure formed in the substrate, adapted for accommodating liquids and comprising at least one binding member adapted for capturing the target molecules and for determining a value indicative of the presence and/or amount of the target molecules, a micro fluidic network interconnecting at least the first structure and the second structure, and an actuator member adapted for effecting a fluid flow between the first structure and the second structure by pressing the flexible cover element against the substrate to selectively close a portion of the micro fluidic network.

L'invention concerne un dispositif comprenant un substrat rigide, un élément de couverture souple recouvrant partiellement au moins ledit substrat, une première structure formée à l'intérieur du substrat, conçue pour accueillir des liquides et libérer le contenu d'une ou plusieurs cellules, spores ou virus, ledit contenu incluant les molécules cibles; une seconde structure formée dans le substrat, conçue pour accueillir des liquides et comprenant au moins un élément de liaison destiné à capturer les molécules cibles et à déterminer une valeur indiquant la présence et/ou la quantité de molécules cibles; un réseau microfluidique reliant au moins la première et la seconde structure; et enfin un élément actionneur conçu pour générer un écoulement de liquide entre la première et la seconde structure par pression de l'élément de couverture souple contre le substrat, de manière à fermer sélectivement une partie du réseau microfluidique.

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