Using structural variation to analyze genomic differences...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2724160

A novel method for prediction of the degree of heterotic phenotypes in plants is disclosed. Structural variation analyses of the genome are used to predict the degree of a heterotic phenotype in plants. In some examples, copy number variation is used to predict the degree of heterotic phenotype. In some methods copy number variation is detected using competitive genomic hybridization arrays. Further, methods for optimizing the arrays are disclosed, together with kits for producing such arrays, as well as hybrid plants selected for development based on the predicted results.

La présente invention concerne une nouvelle méthode de prédiction du degré de phénotypes hétérotiques chez les végétaux. Des analyses de variations structurales du génome sont utilisées pour prédire le degré de phénotype hétérotique chez les végétaux. Dans certains exemples, la variation du nombre de copies est utilisée pour prédire le degré de phénotype hétérotique. Dans certaines méthodes, la variation du nombre de copies est détectée en utilisant des puces d'hybridation génomique compétitive. En outre, des méthodes permettant d'optimiser les puces sont décrites, conjointement avec des trousses pour la production de telles puces, et des végétaux hybrides choisis pour se développer sur la base des résultats prédits.

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