Full length transcript (flt) promoter from figwort mosaic...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01)

Patent

CA 2259210

Use of wild type and modified viral FLt promoters of FMV in the expression of chimeric genes in plant cells. The FLt promoter from FMV is modified with duplicated enhancer domains. The FLt promoter with its single or double enhancer domains is linked to heterologous coding sequences to form chimeric gene constructs. These genes have been shown to be expressed well in plant cells.

L'invention concerne l'utilisation de promoteurs FLt viraux de FMV modifiées et de type sauvage dans l'expression de gènes chimères dans des cellules végétales. Le promoteur FLt issu de FMV est modifé avec des domaines d'amplificateur doublés. Le promoteur FLt avec son unique ou double domaine d'amplificateur est lié à des séquences codantes hétérologues pour former des constructions de gènes chimères. On a démontré que ces gènes étaient bien exprimés dans des cellules de plantes.

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