Genetic screening

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2366942

A method of screening for nucleic acid sequence copy number in a sample genetic material (10), the method including introducing a number of different genetic probes (14) to hybridise with the genetic sample (10). The probes (14) are flanked by the same or substantially the same primer binding sites thereby enabling amplification of sample-bound probes using a single primer pair and thus the screening of different sequence copy numbers at the same time.

L'invention concerne un procédé de criblage d'un nombre de copies de séquences d'acide nucléique dans un matériau génétique échantillon (10), qui consiste notamment à introduire plusieurs sondes (14) génétiques différentes pour hybridisation avec l'échantillon génétique (10). Les sondes (14) sont flanquées par les mêmes, ou sensiblement les mêmes, sites de liaison de l'amorce, ce qui permet d'amplifier les sondes liées à l'échantillon à l'aide d'une paire d'amorce simple et, par conséquent, de cribler différents nombres de copies de séquences en même temps.

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