Rna polymerase mutants with increased thermostability

Details Rna polymerase mutants with increased thermostability Rna polymerase mutants with increased thermostability

C - Chemistry, Metallurgy

12

N

C12N 15/54 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 9/12 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2400049

The present application relates to mutated RNA polymerases from bacteriophages that have increased stability, for example under high temperature conditions. Preferred mutated RNA polymerases according to the invention are mutant RNA polymerases from T7 or SP3 bacteriophages. An especially preferred embodiment of the present invention is a T7 RNA polymerase with a serine to proline amino acid change in the protein at position 633 of the amino acid sequence.

L'invention concerne des ARN polymérases ayant subi des mutations et provenant de bactériophages présentant une stabilité améliorée, par exemple, dans des conditions de température élevées. Les ARN polymérases préférées, ayant subi une mutation, selon l'invention consistent en des ARN polymérases mutantes provenant de bactériophages T7 ou SP3. Un mode de réalisation particulièrement préféré de l'invention consiste en une ARN polymérase T7 dont la protéine présente une modification d'acides aminés sérine à proline au niveau de la position 633 de la séquence aminoacide.

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