Method for in vitro culture of ovarian follicles

Details Method for in vitro culture of ovarian follicles Method for in vitro culture of ovarian follicles

C - Chemistry, Metallurgy

12

N

C12N 5/075 (2010.01) C12Q 1/02 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2429401

The present invention is related to a method for in vitro culture of mammalian ovarian follicles for bioassay purposes, comprising the following subsequent steps: - providing a suitable container for the in vitro culture, - selecting a follicle from an ovary of a mammal, said follicle comprising at least a theca or pre-theca cell, a granulosa cell and an oocyte, - an optional first cultur e step using an attachment prohibiting first medium free from oil, - a second culture step using an attachment promoting second medium free from oil arranged to, and - the retrieval of the matured follicle.

L'invention concerne un procédé de culture in vitro de follicules ovariens de mammifères à des fins de titrage biologique consistant à apporter un récipient approprié pour la culture in vitro, à sélectionner un follicule à partir d'un ovaire d'un mammifère, ledit follicule comprenant au moins une cellule thécale ou préthécale, une cellule de la granulosa et un ovocyte, à réaliser une première culture éventuelle utilisant un premier milieu exempt d'huile empêchant la fixation, à réaliser une seconde culture utilisant un second milieu exempt d'huile favorisant la fixation et à récupérer le follicule mûri.

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