Closed-system multi-stage nucleic acid amplification reactions

Details Closed-system multi-stage nucleic acid amplification reactions Closed-system multi-stage nucleic acid amplification reactions

C - Chemistry, Metallurgy

12

P

C12P 19/34 (2006.01) A21C 3/04 (2006.01) A21C 11/16 (2006.01)

Patent

CA 2587085

The invention is directed to systems, methods and apparatus for carrying out multi-stage amplification reactions, especially under fluidly closed conditions. In one aspect, methods of the invention are carried out in a fluidly closed reaction system that permits the isolation of a portion of a first (or prior) reaction mixture and its use a s a sample or specimen in a second (or subsequent) reaction mixture, thereby substantially avoiding interfering effects that first reaction components may have in the second reaction if both reaction mixture were simply combined together. In the aspect, systems, methods, and apparatus of the invention may be used with any amplification reaction that permits multiple stages of amplification based on the use of nested primers.

L'invention concerne des systèmes, des procédés et des appareils destinés à effectuer des réactions d'amplification à stades multiples, en particulier dans des conditions fermées de circulation de fluide. Suivant un aspect, les procédés selon l'invention sont effectués dans un système de réaction fermé à circulation de fluide, permettant l'isolement d'une portion d'un premier mélange réactionnel (ou d'un mélange antérieur), et son utilisation comme échantillon dans un second mélange réactionnel (ou d'un mélange subséquent), ce qui permet d'éviter sensiblement les effets d'interférence que les composants de la première réaction peuvent avoir dans la seconde réaction, si les deux mélanges réactionnels sont combinés entre eux. Suivant un aspect de l'invention, les systèmes, procédés et appareils de l'invention peuvent être utilisés avec toute réaction d'amplification permettant des stades multiples d'amplification basés sur l'utilisation d'amorces encastrées.

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