Poliovirus chimaeras

Details Poliovirus chimaeras Poliovirus chimaeras

C - Chemistry, Metallurgy

12

N

C12N 15/86 (2006.01) A61K 39/13 (2006.01) C07K 14/005 (2006.01) C07K 14/025 (2006.01) C07K 14/16 (2006.01) C07K 14/295 (2006.01) C07K 14/73 (2006.01) C12N 7/01 (2006.01) C12N 15/43 (2006.01)

Patent

CA 2055613

2055613 9015145 PCTABS00002 A cassette vector suitable for use in constructing poliovirus chimaeras, which vector comprises, under the control of a promoter, a full length infectious cDNA of an attenuated strain of type 1 poliovirus having Sal 1 and Dra 1 sites flanking atigenic site 1 of the poliovirus (I), where the numbers represent the numbers of amino acids of the VP1 capsid protein and X represents intervening nucleotides of DNA, present in sufficient numbers to allow the vector to be digested with both Sal 1 and Dra 1, the said Sal 1 and Dra 1 sites being the only Sal 1 and Dra 1 sites in the vector.

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