Production of secondary metabolites

C - Chemistry – Metallurgy – 12 – M

Patent

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C12M 1/40 (2006.01) C12M 1/12 (2006.01) C12M 1/14 (2006.01)

Patent

CA 2448388

A method of producing a secondary metabolite includes providing an elongate porous substrate with a biofilm of micro-organism and which is arranged with one end of the substrate being at a higher elevation than the other end of the substrate so that the substrate is at an angle to the horizontal. A nutrient solution flows through the substrate, at a rate which is sufficiently low for a nutrient gradient to be established across the biofilm such that the nutrient concentration at a high level along the gradient is sufficiently high to support primary growth of the micro-organism, and the nutrient concentration at a low level along the gradient is sufficiently low to induce secondary growth of the micro-organism. The substrate's angle with the horizontal ensures that any droplets of nutrient permeate forming on the biofilm run towards the lower end of the substrate.

L'invention concerne un procédé de production d'un métabolite secondaire comportant un substrat poreux allongé doté d'un biofilm de micro-organisme et qui est disposé avec une extrémité du substrat placé en élévation supérieure à celle de l'autre extrémité du substrat, de manière à ce que le substrat se trouve à un angle par rapport à l'horizontale. Une solution de nutriment traverse le substrat, à une vitesse assez faible pour qu'un gradient de nutriment soit établi dans le biofilm, de manière à ce que la concentration de nutriment soit de niveau élevé, le gradient suffisamment haut pour soutenir une croissance principale du micro-organisme et la concentration de nutriment à un niveau faible également, le gradient assez bas pour lancer une croissance secondaire du micro-organisme. L'angle du substrat par rapport à l'horizontale garantit que toute goutte du perméat de nutriment se formant sur le biofilm glisse en direction de l'extrémité inférieure du substrat.

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