Method for identifying and/or analysing biological...

Details Method for identifying and/or analysing biological... Method for identifying and/or analysing biological...

G - Physics

01

N

G01N 27/416 (2006.01) C12Q 1/25 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2294627

The invention concerns an improved method for identifying and/or analysing polynucleotides PN present in a conductive liquid medium, using an affinity sensor comprising a multilayer structure semiconductor/insulant/probe = polynucleotides PNc complementary to the medium PN. The method consists in selecting non-marked PNc; polarising the semiconductor; periodically lighting it; measuring the flat band potential variations induced by loading effect phenomenon directly and essentially related to specific pairings between PN and PNc; and finally interpreting the signals collected at the end of the identification and/or analysis of PN. The invention also concerns the device and the sensor used for implementing the method. It is applicable to molecular biology analysis, e.g. diagnosis of viral or genetic diseases.

L'invention a pour but de fournir un procédé perfectionné d'identification et/ou de dosage de polynucléotides PN présents dans un milieu liquide conducteur. Ce procédé consiste à faire intervenir un capteur d'affinité comprenant une structure multicouches semi-conducteur/isolant/sondes = polynucléotides PNc complémentaires aux PN du milieu. Selon ce procédé, on sélectionne des sondes PNc non marquées, on polarise le semi-conducteur, on l'éclaire périodiquement, on mesure les variations du potentiel de bande plate induites par un phénomène d'effet de charge directement et essentiellement lié aux appariements spécifiques entre PN et PNc, et enfin on interprète les signaux recueillis en terme d'identification et/ou de dosage des PN. L'invention concerne également le dispositif et le capteur utile pour la mise en oeuvre de ce procédé. Application: analyse en biologie moléculaire, e.g. diagnostic de maladies virales ou génétiques.

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