Methods and compositions for diagnosis and treatment of...

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G01N 33/569 (2006.01) A61K 38/00 (2006.01) A61K 39/00 (2006.01) A61P 31/16 (2006.01) C07K 5/10 (2006.01) C07K 7/06 (2006.01) C07K 16/10 (2006.01)

Patent

CA 2613749

The invention provides method and compositions for determining the presence and amount of a virus such as HIV-1, HIV-2, Hepatitis B, Hepatitis C, RSV, Rotavirus A, and M. tuberculosis in a sample. Also provided are methods for determining whether a subject is infected with HIV, as well as, the type. The methods involve contacting a sample from the subject with a PDZ polypeptide (PDZ) or other PL binding factor and/or PDZ ligands (PL) and determining whether binding interactions occur between PDZ or other binding factor and PL. Assays for identifying anti-viral and anti-bacterial agents are also provided, as well as, methods for using the compositions to alter PDZ binding to PL in viral or bacterial infected cells.

L'invention concerne une méthode et des compositions permettant de déterminer la présence et la quantité d'un virus tel que le VIH-1, le VIH-2, l'hépatite B, l'hépatite C, le RSV, le rotavirus A, et M. tuberculosis dans un prélèvement. L'invention concerne également des méthodes permettant de déterminer si un sujet est infecté par le VIH, ainsi que le type. Lesdites méthodes consistent à mettre en contact un prélèvement effectué chez un sujet avec un polypeptide PDZ (PDZ) ou un autre facteur de liaison PL et/ou des ligands PDZ (PL), et à déterminer si des interactions de liaison se produisent entre PDZ ou un autre facteur de liaison et PL. L'invention concerne également des dosages d'identification d'agents antiviraux et antibactériens, ainsi que des méthodes d'utilisation des compositions pour altérer la liaison de PDZ à PL dans les cellules virales ou bactériennes infectées.

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