Cryopreservation of cultured epithelial sheets

Details Cryopreservation of cultured epithelial sheets Cryopreservation of cultured epithelial sheets

C - Chemistry, Metallurgy

12

N

C12N 5/02 (2006.01) A01N 1/02 (2006.01)

Patent

CA 2084648

Disclosed is a method of cryopreserving a sheet of living, cultured epthelial cells for use as a skin wound dressing. The method ensures that the integrity of the sheet will be maintained, and it preserves a significant number of cells in mitotically competent, physiologically healthy form. It involves use of a non cell penetrating cryoprotectant and certain freezing and thawing conditions.

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