In-situ cell extraction and assay method

Details In-situ cell extraction and assay method In-situ cell extraction and assay method

G - Physics

01

N

G01N 33/53 (2006.01) C12Q 1/00 (2006.01) C12Q 1/68 (2006.01) G01N 1/38 (2006.01) G01N 33/68 (2006.01) G01N 33/84 (2006.01) G01N 33/88 (2006.01)

Patent

CA 2230423

A method of assaying for an analyte comprises the steps of: i) mixing a sample of cells possibly containing the analyte with a cell lysis reagent to provide a cell lysis fluid, ii) mixing the cell lysis fluid with reagents, including a specific binding partner of the analyte for binding to the analyte, for performing a specific binding assay for the analyte, iii) and mixing the cell lysis fluid with a sequestrant for the cell lysis reagent, whereby the binding of step ii) is performed in the presence of the sequestrant.

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