Equine arteritis virus peptides, antibodies and their use in...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/40 (2006.01) A61K 39/12 (2006.01) C07K 7/08 (2006.01) C07K 14/08 (2006.01) C07K 14/165 (2006.01) C07K 16/10 (2006.01) G01N 33/569 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2181159

A peptide or peptide conjugate is provided comprising one ore mor epitopes capable of producing an immune response against equine arteritis virus in animals characterised in that the epitopes are selected from those present in the amino acid sequence corresponding to amino acid 28 to 137 of equine arteritis virus GL protein (SEQ ID No. 2); the peptide not being equine arteritis virus GL protein as such. The peptides and conjugates have application in vaccines directed against equine arteritis virus, an agent implicated in equine abortion, and as binding agents for use in binding assays, including ELISA assays, for antibodies thereto. Furthermore, antibodies and antisera to the peptides and peptide conjugates may also be used as such binding agents in assays directed at the virus itself.

Cette invention se rapporte à un peptide ou à un conjugué peptidique, comprenant un ou plusieurs épitopes capables de produire une réponse immunitaire contre le virus de l'artérite des équidés. Ce peptide ou conjugué peptidique se caractérise en ce que les épitopes sont choisis parmi ceux qui sont présents dans la séquence d'acides aminés correspondant à l'acide aminé 28 à 137 de la protéine G¿L? du virus de l'artérite équine (SEQ ID No 2); ce peptide n'étant pas la protéine G¿L? du virus de l'artérite équine en tant que telle. Ces peptides et ces conjugués trouvent des applications dans les vaccins dirigés contre le virus de l'artérite équine, lequel est un agent intervenant dans l'avortement des juments pleines, et comme agents de liaison utilisables dans des immunodosages, y compris les méthodes ELISA, pour des anticorps contre ces peptides et conjugués. Des anticorps et des antisérums contre ces peptides et conjugués peptidiques peuvent en outre être utilisés comme agents de liaison dans des immunodosages dirigés sur le virus lui-même.

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