Polymeric hemoglobin mutants

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/12 (2006.01) C07H 17/00 (2006.01) C07K 14/805 (2006.01) C12N 5/10 (2006.01) C12P 21/06 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2344604

Human .beta.-globin mutant polypeptides comprise the amino acid sequence of normal human .beta.-globin modified by (i) the substitution or deletion of Cys at positions (93) and (112), and (ii) the substitution of a Cys for a non-Cys amino acid at one other position in the polypeptide, preferably Ser at position (9). Modified human hemoglobins containing this or other .beta.- globin mutations, and/or an .alpha.-globin mutation, are provided, as well as polymers of such modified hemoglobins. Nucleic acids, vectors and transformed host cells for producing the .beta.-globin mutants are provided.

Selon cette invention, des polypeptides mutants de la .beta.-globine humaine comprennent une séquence d'acides nucléiques de la .beta.-globine humaine normale modifiée (i) par la substitution ou la suppression de Cys dans les positions 93 et 112 et (ii) par la substitution de Cys par un acide aminé non-Cys en une autre position dans le polypeptide, de préférence, par Ser en position 9. L'invention concerne aussi des hémoglobines humaines modifiées contenant ces mutations (ou autres mutations) de la .beta.-globine ainsi que les polymères de ces hémoglobines modifiées. En outre, l'invention concerne des acides nucléiques, des vecteurs et des cellules hôtes modifiées destinées à la fabrication des mutants de la .beta.-globine.

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