Method for protein identification using mass spectrometry data

H - Electricity – 01 – J

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H01J 49/04 (2006.01) G01N 27/62 (2006.01) G01N 33/483 (2006.01) G01N 33/68 (2006.01) H01J 49/40 (2006.01)

Patent

CA 2477151

The present teachings describe methods for matching a query peptide to a database of known peptides based on a mass analysis approach. The methods described herein facilitate rapid, sensitive, and selective identification of an unknown query peptide and provide the ability to develop applications which perform substantially automated high throughput protein identification. The methods described herein also allow for mass spectrometry data for a query peptide to be categorized and weighted according to its quality. Furthermore, the methods described herein provide robust identification of modified query proteins by either anticipating modifications or adjusting for modified peptide masses.

L'invention porte sur des procédés de mise en correspondance de peptides recherchés avec une base de données de peptides connus se basant sur une approche d'analyse de masse. Lesdits procédés permettent une identification rapide, sensible et sélective d'un peptide recherché inconnu et fournissent par-là la possibilité de développer des applications assurant l'identification sensiblement automatique et à haut débit de protéines.

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