Detection of reticulocytes

G - Physics – 01 – N

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G01N 21/64 (2006.01) G01N 15/14 (2006.01) G01N 33/52 (2006.01) G01N 33/80 (2006.01)

Patent

CA 2191096

A process for determining the reticu- locyte population in blood samples, which process includes the use of coriphosphine O to stain reticulocytes and which process is partic- ularly suitable for detection by flow cytometry techniques. The optics (10) of a flow cytome- ter useful in the process includes an argon ion laser (12) as a light source which illuminates a blood sample flowing through a flow cell (14). Scattered light and fluorescence fall upon a dichroic mirror (22) which serves to reflect the right-angle scattered light (24) and trans- mit the fluorescence (26). A dichroic mirror (30) serves to reflect green fluorescence (32) and transmit red fluorescence (38).

Procédé de détermination de la population de réticulocytes dans des échantillons de sang, consistant à utiliser de la corisphorine O afin de marquer les réticulocytes, ledit procédé étant particulièrement approprié à la détection à l'aide de techniques de cytométrie de flux. L'optique (10) d'un cytomètre de flux utile dans ce procédé comprend un laser à l'argon ionisé (12) en tant que source de lumière illuminant un échantillon de sang circulant à travers une cellule (14) de flux. La lumière diffusée et la fluorescence tombent sur un miroir dichroïque (22) qui sert à réfléchir la lumière (24) diffusée à angle droit et à transmettre la fluorescence (26). Un miroir dichroïque (30) sert à réfléchir la fluorescence verte (32) et à transmettre la fluorescence rouge (38).

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