Methods for stable transformation of plants

Details Methods for stable transformation of plants Methods for stable transformation of plants

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) A01H 4/00 (2006.01) A01H 5/00 (2006.01)

Patent

CA 2418056

Multiple shoot structures are induced from plant tissues (e.g., shoot apices or axillary buds on an artificial medium) to produce multiple shoot cultures. These multi-shoot cultures are then transformed by known transformation methods. Plants are subsequently regenerated from the transformed cells. Crops that may be efficiently transformed by this method include plants normally recalcitrant to transformation such as sugar beet, sunflower, soybean, cotton, tobacco, tomato, peanuts, melons, watermelon, squash, Brassica, and pepper.

De multiples structures de pousses sont extraites de tissus végétaux (p. ex. apex de tige ou bourgeons axillaires sur milieu artificiel) en vue de produire des cultures à pousses multiples. Ces cultures à pousses multiples sont ensuite transformées à l'aide de procédés de transformation connus. Des végétaux sont ensuite régénérés à partir des cellules transformées. Les cultures pouvant être transformées efficacement à l'aide de ce procédé incluent des végétaux qui résistent normalement aux transformations, tels que betterave sucrière, tournesol, soya, coton, tabac, tomate, cacahuètes, melons, pastèque, courge, Brassica et poivre.

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