Method and device for combined enrichment, processing and...

G - Physics – 01 – N

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G01N 1/28 (2006.01) G01N 1/36 (2006.01) G01N 1/31 (2006.01)

Patent

CA 2066732

2066732 9103720 PCTABS00003 The invention concerns a device and an apparatus for the processing of cytological specimens (1) according to histological principles. The device comprises a preferably cone-shaped cup (2) with a closed bottom part, side-walls defining an upper aperture with a surface area larger than that of the said bottom part, as well as a perforated membrane (3) adapted to cover the said aperture of the cup for retaining the cytological specimens, the hydraulic conductance of the membrane being sufficient to allow the exchange of processing fluids when the cup (2) is inserted in the tissue processing apparatus, the shape of the cup promoting such exchange, and means for attaching the said membrane to the cup. In the method the cup with specimens is inserted in the tissue processor with the net in a vertical position, after processing and paraffination the cup is placed on a cold plate with the net in the horizontal position allowing the specimens to sedimentate to form an enriched layer at the bottom of the cup. The solid paraffin block with enriched layer is thereafter removed from the cup and cut on a microtome.

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