Assay for carbohydrate-free transferrin

Details Assay for carbohydrate-free transferrin Assay for carbohydrate-free transferrin

G - Physics

01

N

G01N 33/68 (2006.01) G01N 33/98 (2006.01)

Patent

CA 2294992

The present invention provides a method for the determination of carbohydrate- free transferrin in a body fluid for use in the assessment of alcohol consumption, said method comprising (a) contacting a sample of said body fluid with a carbohydrate-binding ligand, to bind any carbohydrate or carbohydrate- containing moieties in said sample to said ligand; (b) separating a fraction not binding to said ligand and (c) determining the content of transferrin in said fraction. Also provided are kits for carrying out such a method.

La présente invention concerne une méthode de détermination de transferrine exempte d'hydrates de carbone dans un liquide organique, la méthode pouvant s'utiliser pour déterminer la consommation d'alcool et consistant: (a) à mettre en contact un échantillon du liquide organique avec un ligand de liaison d'hydrates de carbone, pour lier au ligand tout hydrate de carbone ou toute fraction contenant des hydrates de carbone présents dans l'échantillon; (b) à extraire une fraction ne se liant pas au ligand; (c) à déterminer la teneur en transferrine de la fraction. L'invention concerne également des trousses permettant la mise en oeuvre de cette méthode.

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