Pcr elbow determination using curvature analysis of a double...

Details Pcr elbow determination using curvature analysis of a double... Pcr elbow determination using curvature analysis of a double...

G - Physics

06

F

G06F 19/10 (2011.01) C12P 19/34 (2006.01) G06F 17/10 (2006.01) G01N 33/557 (2006.01)

Patent

CA 2572440

Systems and methods for determining characteristic transition values such as elbow values in sigmoid or growth-type curves, such as the cycle threshold (Ct) value in PCR amplification curves. A double sigmoid function with parameters determined by a Levenberg-Marquardt (LM) regression process is used to find an approximation to a curve that fits a PCR dataset. Once the parameters have been determined, the curve can be normalized using one or more of the determined parameters. After normalization, the normalized curve is processed to determine the curvature of the curve at some or all points along the curve, e.g., to produce a dataset or plot representing the curvature v. the cycle number. The cycle number at which the maximum curvature occurs corresponds to the Ct value. The Ct value is then returned and may be displayed or otherwise used for further processing.

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