Holder for pcr sample collection and preparation

B - Operations – Transporting – 01 – L

Patent

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Details

B01L 3/00 (2006.01) G01N 1/02 (2006.01)

Patent

CA 2527304

A holder for PCR sample collection and preparation and method of using which has a buffer container housing removably connected to a plunger housing. A swab attached to an end of a plunger collects a sample of a specimen to be analyzed for biological warfare agents. The swab and plunger are inserted into the plunger housing, a buffer container is positioned inside the buffer container housing and the buffer container housing and plunger housing are attached. A buffer passes through the swab and elutes off the sample and the sample mixes with a reagent. The prepared sample loads into a reaction tube, by a whipping action, for analysis.

La présente invention concerne, d'une part une éprouvette pour recueillir et préparer des échantillons pour amplification par réaction en chaîne de la polymérase, et d'autre part un procédé d'utilisation utilisant un logement de récipient tampon relié à un logement de piston. Un porte-coton attaché à une extrémité d'un piston recueille un échantillon d'un spécimen dans lequel on recherche par des analyses des agents de guerre bactériologique. Le porte-coton et le piston sont insérés dans le logement du piston. Un récipient tampon est placé à l'intérieur du logement du récipient tampon, le logement du récipient tampon et le logement du piston étant solidaires. Un agent tampon traverse le porte-coton et chasse par élution l'échantillon qui se mélange à un réactif. L'échantillon préparé se charge dans un tube de réaction, par fouettage, aux fins d'analyse.

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